Platelet Factor 4 human Biotin-labeled
Properties*
| Product # | Platelet Factor 4 human Biotin labeld (PF4-h-B) |
| Species | human |
| Source | Thrombocytes |
| Mol wt. | 7.8 kDa (monomer) |
| Label | Biotin-XX, SSE |
| UniProt # | P02776 |
| Purity | > 98% (SDS-PAGE, silver staining) |
| Product sizes | 100 µg, 200 µg, 1mg (different sizes are available on request) |
| Quality control | PF4/Heparin-ELISA (HIT-Test)**, PF4-ELISA, Biotin-ELISA, SDS-Page, Western Blot, N-terminal sequencing and MALDI-TOF-MS |
| Physical form | Lyophilized in PBS (0.22 µm filtered), carrier free (different buffers are available on request) |
| Reconstitution | Reconstitute carefully in A. dest. (1µl/µg PF4). Adjust the protein concentration with PBS. Do not vortex. |
| Shipping | Ambient temperature |
| Storage | Store dark in working aliquots at -20°C to -80°C. Avoid repeated freezing and thawing. |
| Stability | Lyophilisate is stable for at least 12 month. |
Description
Platelet Factor 4 (PF4; also known as CXCL4) is synthesized in megakaryocytes and platelets. The monomer of the chemokine consists of 70 amino acids resulting in a molecular weight of 7.8 kDa. PF4 is biologically active in the tetrameric form, promotes blood coagulation and is also important in wound healing and inflammation. PF4, together with heparin (PF4-heparin complex) is an important antigen of antibodies inducing heparin- induced thrombocytopenia (HIT). Purified PF4 is used in several immuno assays for the detection of HIT antibodies. Labeling of PF4 with Biotin-XX, SSE (14-atom spacer improves binding of antibodies, avidin and streptavidin) enables effective detection of PF4 in these assays.
* Please note that the properties of this product (structure, antigenicity, function etc.) may alter under different experimental conditions. If changes (buffers, pH etc.) are made, the responsibility is transferred from the seller to the customer. The material is neither intended nor certified for human or animal therapeutic use.
** The production of PF4 and its quality control is performed in collaboration with the Institute of Immunology and Transfusion Medicine, Department of Transfusion Medicine, University Medicine Greifswald.
List of prices: 
Request to: info[at]chromatec.de
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