Platelet Factor 4 Variant 1 human recombinant

Properties

Product # Platelet Factor 4 Variant 1 human recombinant (PF4-V1-hr)
Species human
Source Escherichia coli
Mol wt 8.7 kDa (monomer, including 6 x His tag)
UniProt # P10720
Purity > 95% (SDS-PAGE)
AA sequence MHHHHHHEAEEDGDLQCLCVKTTSQVRPRHITSLEV
IKAGPHCPTAQLIATLKNGRKICLDLQALLYKKIIKEHLES
Product sizes 50 µg, 100 µg, 200 µg (different sizes are available on request)
Quality control SDS-PAGE, Western Blot, N-terminal sequencing and MALDI-TOF-MS
Physical form Lyophilized in PBS (0.22 µm filtered), carrier free
(different buffers are available on request)
Reconstitution Reconstitute carefully in A. dest. (1 µl/µg PF4).
Adjust the protein concentration with PBS. Do not vortex.
Shipping Ambient temperature
Storage Store dark in working aliquots at -20°C to -80°C.
Avoid repeated freezing and thawing.
Stability Lyophilisate is stable for at least 12 month.

Description

Platelet Factor 4 (PF4; also known as CXCL4) is synthesized in megakaryocytes and platelets. The mature protein of PF4 Variant 1 (PF4-V1 or CXCL4L1) differs from authentic PF4 only in three carboxy-terminal amino acids. PF4-V1 was shown to exhibit different properties and cellular functions compared to PF4, as well as a different subcellular distribution (38 % difference in the amino acid sequence of the signal peptide). PF4-V1-hr (ChromaTec GmbH) is produced recombinantly in E. coli and is affinity purified via an N-terminal 6 x His-tag. The monomer of PF4-V1-hr including the 6 x His-tag has a molecular weight of 8.7 kDa.

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Datasheet:  

SDS-Page